產品英文名稱:Insulin Ultrasensitive ELISA Kit 產品中文名稱:人胰島素超靈敏ELISA檢測試劑盒 人胰島素介紹: 胰島素是由胰島β細胞受內源性或外源性物質如核糖、乳糖、葡萄糖、胰 高血糖素、精氨酸等的刺激由胰腺朗格漢斯小島所分泌的一種蛋白質激素,由A、B鏈組成,共含51個氨基酸殘基。胰島素是機體內*降低血糖的激素,同時促進糖原、脂肪、蛋白質合成。外源性胰島素主要用來進行糖尿病治療,糖尿病患者早期使用胰島素和*抗氧化劑如(口服蝦青素、注射用硫辛酸等)有望出現較長時間的蜜月期,胰島素注射不會有成癮和依賴性。 § 檢測原理:ALPCO人胰島素超靈敏ELISA是一個夾層式免疫檢測試劑盒。96孔微孔板涂有一層胰島素特異性單克隆抗體。將標準物,對照品和樣品一并添加到微型板小孔中與檢測抗體混合。然后將微孔板放在搖床上溫育1小時,700-900轉/分鐘。*次溫育結束,用清洗緩沖液清洗小孔并烘干。添加TMB作用底物,放在微孔板搖床上第二次溫育15分鐘,700-900轉/分鐘。第二次溫育結束,添加反應終止液,調節分光光度計到450nm測定OD值。由于發光強度與樣品中胰島素含量成比例,因此可以根據OD值確定胰島素含量。 § 檢測過程:各添加25 μl標準物,對照物和樣品→加100µl檢測抗體→微孔板搖床溫育1小時,700-900rpm→清洗緩沖液清洗6次→加100µl TMB作用物→微孔板搖床溫育15分鐘,700-900rpm→添加100µl反應終止液→分光光度計450nm讀數。 Ultrasensitive Insulin ELISA英文簡述: INTENDED USE The ALPCO Ultrasensitive Insulin ELISA is designed for the quantitative determination of insulin in human serum and plasma. PRINCIPLE OF THE ASSAY The ALPCO Ultrasensitive Insulin ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for insulin. The standards, samples, and the control are added to the microplate wells with the Detection Antibody. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700-900 rpm. Once the second incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of insulin in the sample. CALCULATION OF RESULTS Construct a standard curve from the standards. The Zero Standard should be used as a blank with its average value subtracted from each well. It is recommended to use a software program to calculate the standard curve and to determine the concentration of the samples. The ALPCO Ultrasensitive Insulin ELISA is a ligand binding assay, with responses exhibiting a sigmoidal relationship to the analyte concentration. Currently accepted reference models for such curves use a 4 or 5 parameter logistic (pl) fit, as these models optimize the accuracy and precision across a greater range. Although cubic spline and other models are acceptable methods, they generally show less intra-assay accuracy and precision at the low and high ends of the range. 相關產品: 產品名稱
| 貨號 | 樣本容量 | 規格 | Insulin ELISA | 80-INSHU-E01 | 25µl | 96T/盒 | Insulin Ultrasensitive ELISA | 80-INSHUU-E01 | 25µl | 96T/盒 | Insulin (Synthetic) ELISA | 80-SINHU-E01 | 25µl | 96T/盒 | Insulin (Mouse) ELISA | 80-INSMS-E01 | 10µl | 96T/盒 | Insulin (Mouse) High Range ELISA | 80-INSMSH-E01 | 5µl | 96T/盒 | Insulin (Mouse) Ultrasensitive ELISA(5µl) | 80-INSMSU-E01 | 5µl | 96T/盒 | Insulin (Mouse) Ultrasensitive ELISA(25µl) | 80-INSMSU-E01 | 25µl | 96T/盒 | Insulin (Rat) ELISA | 80-INSRT-E01 | 10µl | 96T/盒 | Insulin (Rat) High Range ELISA | 80-INSRTH-E01 | 5µl | 96T/盒 | Insulin (Rat) Ultrasensitive ELISA(5µl) | 80-INSRTU-E01 | 5µl | 96T/盒 | Insulin (Rat) Ultrasensitive ELISA(25µl) | 80-INSRTU-E01 | 25µl | 96T/盒 | Insulin (Bovine) ELISA | 80-INSBO-E01 | 25µl | 96T/盒 | Insulin (Ovine) ELISA | 80-INSOV-E01 | 25µl | 96T/盒 | Insulin (Porcine/Canine) ELISA | 80-INSPO-E01 | 25µl | 96T/盒 | C-Peptide ELISA | 80-CPTHU-E01.1 | 25µl | 96T/盒 | C-Peptide (Mouse) ELISA | 80-CPTMS-E01 | 10µl | 96T/盒 | C-Peptide (Rat) ELISA | 80-CPTRT-E01 | 10µl | 96T/盒 | Proinsulin (Mouse) ELISA | 80-PINMS-E01 | 10µl | 96T/盒 | Proinsulin ELISA | 80-PINHU-E01 | 50µl | 96T/盒 | Proinsulin (Rat) ELISA | 80-PINRT-E01 | 10µl | 96T/盒
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重慶市華雅干細胞技術有限公司 |