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Anti-Vimentin antibody [2D1] (ab125089)抗波形蛋白抗體產品信息:
Mouse monoclonal [2D1] to Vimentin
Reacts with: Mouse, Rat, Cow, Human, Pig
Recombinant full length purified protein corresponding to Human Vimentin expressed in E.coli.
Properties
Concentration 100 µl at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab125089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
WB | WB: 1/10000. Predicted molecular weight: 54 kDa. |
ICC/IF | ICC/IF: 1/1000. |
Flow Cyt | Flow Cyt: Use 0.1µg for 106 cells. |
Target
Database links
Alternative names
Anti-Vimentin antibody [2D1] images
Flow Cytometry - Anti-Vimentin antibody [2D1] (ab125089)
Overlay histogram showing HeLa cells stained with ab125089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125089, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [2D1] (ab125089)
ab125089, at 1/1000, staining Vimentin in mixed neuron/glial cultures by Immunofluorescence (green).Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments
Western blot - Anti-Vimentin antibody [2D1] (ab125089)
Anti-Vimentin antibody [2D1] (ab125089) at 1/10000 dilution + HeLa cell line lysate
developed using the ECL technique
Predicted band size : 54 kDa
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