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Anti-FOXP2 antibody (ab16046)抗FOXP2蛋白抗體產品信息:
Rabbit polyclonal to FOXP2
Reacts with: Mouse, Rat, Human
Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human FOXP2.
Read Abcam's proprietary immunogen policy
(Peptide available as ab16278.)
Properties
Concentration 100 µg at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab16046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
WB | WB: 1/1000. Can be blocked with Human FOXP2 peptide (ab16278). (see abreview) |
IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. PubMed: 19490899 |
IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
ICC/IF | ICC/IF: Use at an assay dependent dilution. |
IHC-P | IHC-P: 1/50 - 1/2500. PubMed: 19136970 |
Target
Database links
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Alternative names
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Anti-FOXP2 antibody images
Immunocytochemistry/ Immunofluorescence - FOXP2 antibody (ab16046)
Mouse spinal cord was fixed in paraformaldehyde, blocked in 1% BSA for 30 minutes then incubated with ab16046 at 1/8000 dilution for 18 hours. This image was submitted as part of a review by Jeremy Dasen.
Western blot - FOXP2 antibody (ab16046)
This image is courtesy of an anonymous Abreview
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP2 antibody (ab16046)
Image courtesy of Human Protein Atlas
ab16046 staining FOXP2 in human testis. Paraffin embedded human testis tissue was incubated with ab16046 (1/600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab16046 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Western blot - FOXP2 antibody (ab16046)
Anti-FOXP2 antibody (ab16046) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 56 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - FOXP2 antibody (ab16046)This image is courtesy of an anonymous Abreview
ab16046 staining FOXP2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde, permeabilized by 0.4% Triton X and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/8000) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.See Abreview
Immunocytochemistry/ Immunofluorescence - FOXP2 antibody (ab16046)
ICC/IF image of ab16046 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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