咨詢： （重慶） （貴陽） Anti-ZO1 tight junction protein antibody (ab59720)抗ZO1緊密連接蛋白抗體產品信息： - Product name：Anti-ZO1 tight junction protein antibodySee all ZO1 tight junction protein primary antibodies ...
- Description
Rabbit polyclonal to ZO1 tight junction protein - Specificityab59720 recognises both ZO1 tight junction alpha-minus and alpha-plus isoforms. The protein is abundant in endothelial cells and the highly specialized epithelial junctions. Although we have an image for IHC-Fr, customers report variable results in this application. If you would like to use in IHC-Fr then we suggest using ethanol instead of acetone. Although we have references for IHC-P, customers report variable results in this application. IHC-Fr and IHC-P are therefore not covered by our Abpromise guarantee.
- Tested applicationsICC/IF, Flow Cyt, IHC (PFA fixed), WB, IHC-P more details
- Species reactivity
Reacts with: Mouse, Chicken, Dog, Human, Pig - Immunogen
Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein
Properties Applications Our Abpromise guarantee covers the use of ab59720 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. | Application | Notes |
|---|
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. | | Flow Cyt | Flow Cyt: 1/50. | | IHC (PFA fixed) | IHC (PFA fixed): Use at an assay dependent concentration. PubMed: 21526120 | | WB | WB: 1/50. Predicted molecular weight: 194 kDa. | | IHC-P | IHC-P: Use at an assay dependent concentration. |
Target - FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
- Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
- Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain. - DomainThe second PDZ domain mediates interaction with GJA12.
- Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRJ. - Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
- Information by UniProt
Database links - Entrez Gene: 7082 Human
- Entrez Gene: 21872 Mouse
- Omim: 601009 Human
- SwissProt: Q07157 Human
- SwissProt: P39447 Mouse
- Unigene: 510833 Human
- Unigene: 4342 Mouse
Alternative names - Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
Anti-ZO1 tight junction protein antibody images Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720) ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.  Immunocytochemistry/ Immunofluorescence - ZO1 tight junction protein antibody (ab59720) ICC/IF image of ab59720 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.  Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)This image is courtesy of an anonymous Abreview Immunofluorescence analysis of HUVEC cells, staining ZO1 tight junction protein with ab59720.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)Image from Kokkinopoulos I et al., PLoS One. 2011 Apr 22;6(4):e18921. Fig 6.; doi:10.1371/journal.pone.0018921; April 22, 2011, PLoS ONE 6(4): e18921. Immunofluorescence analysis of murine peripheral retinal pigmented epithelium (RPE) cells, staining ZO1 tight junction protein with ab59720.
Cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were blocked in 10% BSA and 0.5% Triton-X 100 for 2 hours at room temperature before being incubated with primary antibody (1/100) in blocking solution overnight at room temperature. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.
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Polyglutamylated100ul4274Tubulin抗體一抗 Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗 Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗體一抗 Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗體一抗 Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗體一抗 Abcam ab12964Rabbit polyclonal to Corticotropin Releasing Factor Receptor 250ug5029Corticotropin Releasing Factor Receptor 2hystemcell一抗 Abcam ab1297Rabbit polyclonal to SMC3 （Agarose）50ug3996SMC3hystemcell一抗 Abcam ab12974Rabbit polyclonal to EMR350ug3996EMR3hystemcell一抗 Abcam ab12977Rabbit polyclonal to Endothelin A Receptor50ug4773Endothelin A Receptorhystemcell一抗 Abcam ab1298Mouse monoclonal [mAbcam 1298] to Histone H3 （di methyl K9）100ug3996Histone H3hystemcell一抗 Abcam ab12986Rabbit polyclonal to Estrogen Related Receptor beta50ug3996Estrogen Related Receptor betahystemcell一抗 Abcam ab12989Rabbit polyclonal to ETL50ug3996ETLhystemcell一抗 Abcam ab1299Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker100ug3996GM130hystemcell一抗 Abcam ab12990Rabbit polyclonal to FPR150ug4940FPR1hystemcell一抗 Abcam ab12994Rabbit polyclonal to Frizzled 650ug4940Frizzled 6hystemcell一抗 Abcam ab13001Rabbit polyclonal to GALR150ug3996GALR1hystemcell一抗 Abcam ab13003Rabbit polyclonal to GALR350ug4940GALR3hystemcell一抗 Abcam ab13004Rabbit polyclonal to Gastric Inhibitory Polypeptide Receptor50ug4607Gastric Inhibitory Polypeptide Receptorhystemcell一抗 Abcam ab13008Aurora A peptide100ug2010Aurora A蛋白/多肽多肽 Abcam ab1301Goat polyclonal to LASP1100ug3996LASP1hystemcell一抗 Abcam ab13011Rabbit polyclonal to GnRHR50ug4940GnRHRhystemcell一抗 Abcam ab13015Rabbit polyclonal to HRH325ug3996HRH3hystemcell一抗 Abcam ab13016Rabbit polyclonal to HUNK50ug3996HUNKhystemcell一抗 Abcam ab1302Goat polyclonal to CrkL100ug3996CrkLhystemcell一抗 Abcam ab13022Rabbit polyclonal to LPHN350ug4940LPHN3hystemcell一抗 Abcam ab13025Rabbit polyclonal to GPR10850ug4940GPR108hystemcell一抗 Abcam ab13026Rabbit polyclonal to EDG250ug3996EDG2hystemcell一抗 Abcam ab13027Rabbit polyclonal to EDG225ug3996EDG2hystemcell一抗 Abcam ab13028Rabbit polyclonal to EDG450ug3996EDG4hystemcell一抗 Abcam ab13033Rabbit polyclonal to MC1 Receptor50ug4940MC1 Receptorhystemcell一抗 Abcam ab13034Rabbit polyclonal to MC4 Receptor50ug3996MC4 Receptorhystemcell一抗 Abcam ab13037Rabbit polyclonal to Metabotropic Glutamate Receptor 150ug3996Metabotropic Glutamate Receptor 1hystemcell一抗 Abcam ab1304Mouse monoclonal [10] to Vascular Endothelium100ug3996Vascular Endotheliumhystemcell一抗 Abcam ab13040Rabbit polyclonal to Metabotropic Glutamate Receptor 250ug3996Metabotropic Glutamate Receptor 2hystemcell一抗 Abcam ab13041Rabbit polyclonal to Metabotropic Glutamate Receptor 350ug3996Metabotropic Glutamate Receptor 3hystemcell一抗 Abcam ab13042Rabbit polyclonal to Metabotropic Glutamate Receptor 750ug3996Metabotropic Glutamate Receptor 7hystemcell一抗 Abcam ab13046Mouse monoclonal [CYT3-10] to Cytohesin 3100ul3996Cytohesin 3hystemcell一抗 Abcam ab13048Mouse monoclonal [23A8] to RAC50ug3996RAChystemcell一抗 Abcam ab13049Mouse monoclonal [ARAN1] to Ran50ul3996Ranhystemcell一抗 Abcam ab1305Goat polyclonal to Smad2100ug3996Smad2hystemcell一抗 Abcam ab13051Rabbit polyclonal to Oxytocin Receptor50ug5029Oxytocin Receptorhystemcell一抗 Abcam ab13057Mouse monoclonal [B4F8] to RASGAP50ug3996RASGAPhystemcell一抗 Abcam ab13058Mouse monoclonal [24E4] to REM100ug3885REMhystemcell一抗 Abcam ab13059Mouse monoclonal [11D1] to proBNP200ug3996proBNPhystemcell一抗 Abcam ab13065Rabbit polyclonal to Muscarinic Acetylcholine Receptor M450ug3996Muscarinic Acetylcholine Receptor M4hystemcell一抗 Abcam ab13066Rabbit polyclonal to NPFF1 Receptor50ug3996NPFF1 Receptorhystemcell一抗 Abcam ab1307Goat polyclonal to FOXP2100ug3996FOXP2hystemcell一抗 Abcam ab13071Rabbit polyclonal to Olfactory Receptor LS18974450ug4940Olfactory Receptor LS189744hystemcell一抗 Abcam ab13073Rabbit polyclonal to OR2H350ug3996OR2H3hystemcell一抗 Abcam ab13074Rabbit polyclonal to Mu Opioid Receptor50ug3996Mu Opioid Receptorhystemcell一抗 Abcam ab13078Rabbit polyclonal to Parathyroid Hormone Receptor 150ug3996PTHR 1hystemcell一抗 Abcam ab1308Goat polyclonal to ARFGAP3100ug3996ARFGAP3hystemcell一抗 Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗體一抗 Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗體一抗 Abcam ab11463Mouse monoclonal [BRIC 203] to Blood Group Kell Antigen100ug3996Blood Group Kell Antigenhystemcell一抗 Abcam ab11464Rat monoclonal [HEK/1/85a] to CCR5100ug3996CCR5hystemcell一抗 Abcam ab11465Rat monoclonal [HEK/1/85a] to CCR5 （Biotin）50ug3996CCR5hystemcell一抗 Abcam ab11466Rat monoclonal [HEK/1/85a] to CCR5 （FITC）100ug4940CCR5hystemcell一抗 Abcam ab11467Hamster monoclonal [ GL3 ] to TCR gamma+TCR delta （FITC）100ug3996TCR g/dhystemcell一抗 Abcam ab1147Rabbit polyclonal to CSEN100ul3996DREAMhystemcell一抗 Abcam ab11470Mouse monoclonal [P2W7] to Integrin alpha V50ug3996Integrin alpha Vhystemcell一抗 Abcam ab11472Rabbit polyclonal to LIN7100ul3996LIN7hystemcell一抗 Abcam ab11473Rabbit polyclonal to MAGI150ul3996MAGI1hystemcell一抗 Abcam ab11476Sheep polyclonal to MAGI350ug3996MAGI3hystemcell一抗 Abcam ab11477Histone H3 peptide - phospho S10100ug2010Histone H3蛋白/多肽多肽 Abcam ab11478Hamster monoclonal [GL3] to TCR gamma+TCR delta （Phycoerythrin）250ul3996TCR g/dhystemcell一抗 Abcam ab1148Rabbit polyclonal to ESE1100ul3996ESE1hystemcell一抗 Abcam ab11480Rat monoclonal [KT50] to TCR V alpha 8 （Biotin）500ul5029TCR V alpha 8hystemcell一抗 Abcam ab11482Rat monoclonal [TR310] to TCR V beta 7100ug3996TCR V beta 7hystemcell一抗 Abcam ab11483Rat monoclonal [TR310] to TCR V beta 7 （FITC）100ug3996TCR V beta 7hystemcell一抗 Abcam ab11484Mouse monoclonal [13B9] to Calcitonin （HRP）200ug3996Calcitoninhystemcell一抗 Abcam ab11485Rat monoclonal [TR310] to TCR V beta 7 （Phycoerythrin）250ul3996TCR V beta 7hystemcell一抗 Abcam ab11488Rat monoclonal [KJ16] to TCR V beta 8.1+ 8.2100ug3996TCR V beta 8.1+8.2hystemcell一抗 Abcam ab1149Rabbit polyclonal to IKAP100ul3996IKAPhystemcell一抗 Abcam ab11490Mouse monoclonal [18B7] to Calcitonin100ug3996Catacalcinhystemcell一抗 Abcam ab11493Mouse monoclonal [16B5 ] to Calcitonin250ug3996Calcitoninhystemcell一抗 Abcam ab11495Rat monoclonal [KT10b] to TCR V beta 10b2ml3996TCR V beta 1bhystemcell一抗 Abcam ab11497Mouse monoclonal [24B2 ] to Calcitonin500ug3885Calcitoninhystemcell一抗 Abcam ab11499Rat monoclonal [KT10b] to TCR V beta 10b （Biotin）500ul3996TCR V beta 1bhystemcell一抗 Abcam ab1150Rabbit polyclonal to IKAP100ul3996IKAPhystemcell一抗 Abcam ab11501Rabbit polyclonal to Nephronophthisis50ug3996Nephronophthisishystemcell一抗 Abcam ab11503Goat polyclonal to Osteopontin25ug3996Osteopontinhystemcell一抗 Abcam ab11504Rat monoclonal [KT12] to TCR V beta 12 2ml3996TCR V beta 12hystemcell一抗 Abcam ab11505Mouse monoclonal [G3] to CD3 zeta （FITC）500ul3996TCR zeta chainhystemcell一抗 Abcam ab11506Mouse monoclonal [15F11] to gamma Catenin100ul3996gamma Cateninhystemcell一抗 Abcam ab11507Rabbit polyclonal to delta 1 Catenin50ul3996delta 1 Cateninhystemcell一抗 Abcam ab11508Mouse monoclonal [6H11] to delta 1 Catenin50ul3996delta 1 Cateninhystemcell一抗 Abcam ab11509Mouse monoclonal [SLP-32] to MAGI350ul3996SLIPRhystemcell一抗 Abcam ab1151Rabbit polyclonal to IKK gamma100ul3996IKK gammahystemcell一抗 更多詳情，請： 重慶市華雅干細胞技術有限公司 |
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